View source for MOPS

{{Other uses|Mops (disambiguation){{!}}Mops}}
{{Chembox
| Verifiedfields = changed
| Watchedfields  = changed
| verifiedrevid  = 442344970
| ImageFile      = Mops is.svg
| PIN            = 3-(Morpholin-4-yl)propane-1-sulfonic acid
| OtherNames     = 3-(''N''-Morpholino)propanesulfonic acid,<br />3-Morpholinopropanesulfonic acid,<br />3-''N''-Morpholino propansulfonic acid,<br />4-Morpholinepropanesulfonic acid
| Section1       = {{Chembox Identifiers
| CASNo_Ref = {{cascite|correct|??}}
| CASNo = 1132-61-2
| CASNo_Comment = (free acid)
| CASNo2_Ref = {{cascite|unknown|CAS}}
| CASNo2 = 71119-22-7
| CASNo2_Comment = (sodium salt)
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = 273BP63NV3
| PubChem = 70807
| SMILES = C1COCCN1CCCS(=O)(=O)O
| PubChem2 = 2723950
| ChemSpiderID_Ref = {{chemspidercite|changed|chemspider}}
| ChemSpiderID = 63972
| InChI = 1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
| InChIKey = DVLFYONBTKHTER-UHFFFAOYAN
| StdInChI_Ref = {{stdinchicite|changed|chemspider}}
| StdInChI = 1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
| StdInChIKey_Ref = {{stdinchicite|changed|chemspider}}
| StdInChIKey = DVLFYONBTKHTER-UHFFFAOYSA-N
}}
| Section2       = {{Chembox Properties
| C=7|H=15|N=1|O=4|S=1
| Appearance =
| Density =
| MeltingPt =
| BoilingPt =
| Solubility =
}}
| Section3       = {{Chembox Hazards
| GHSPictograms = {{GHS07}}
| GHSSignalWord = Warning
| HPhrases = {{H-phrases|315|319|335}}
| PPhrases = {{P-phrases|261|264|271|280|302+352|304+340|305+351+338|312|321|332+313|337+313|362|403+233|405|501}}
| MainHazards =
| FlashPt =
| AutoignitionPt =
| ExternalSDS =
}}
}}
'''MOPS''' ('''3-(''N''-morpholino)propanesulfonic acid''') is a [[buffer solution|buffer]] introduced in the 1960s, one of the twenty [[Good's buffers]]. It is a structural analog to [[MES (buffer)|MES]],<ref>{{cite journal|doi=10.1021/bi00866a011|title=Hydrogen Ion Buffers for Biological Research|year=1966|last1=Good|first1=Norman E.|last2=Winget|first2=G. Douglas|last3=Winter|first3=Wilhelmina|last4=Connolly|first4=Thomas N.|last5=Izawa|first5=Seikichi|last6=Singh|first6=Raizada M. M.|journal=Biochemistry|volume=5|pages=467–77|pmid=5942950|issue=2}}</ref> and like MES, its structure contains a [[morpholine]] ring. [[HEPES]] is a similar [[pH]] buffering compound that contains a [[piperazine]] ring. With a [[pKa|p''K''<sub>a</sub>]] of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
==Applications==
MOPS is frequently used as a [[buffering agent]] in [[biology]] and [[biochemistry]]. It has been tested and recommended for [[polyacrylamide gel electrophoresis]].<ref>{{cite journal|doi=10.1016/0003-2697(81)90178-0|pmid=6278979|title=A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH|first2=ME|year=1981|last2=Hodes|last1=Thomas|first1=J|journal=Analytical Biochemistry|volume=118|issue=1|pages=194–6}}</ref> Usage above 20 mM in mammalian [[cell culture]] work is not recommended.<ref>{{cite journal|doi=10.1126/science.174.4008.500|pmid=5110427|title=Buffer Combinations for Mammalian Cell Culture|year=1971|last1=Eagle|first1=H.|journal=Science|volume=174|issue=4008|pages=500–3|bibcode=1971Sci...174..500E|s2cid=29876583}}</ref> MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.<ref>{{cite web |url=https://bostonbioproducts.com/products/mops-buffer-1-m-ph-74-bbm-74 |title=Boston BioProducts - MOPS Buffer (1 M, pH 7.4)}}</ref>
==See also==
*[[CAPS (buffer)|CAPS]]
*[[HEPPS (molecule)|HEPPS]]
*[[Tris]]
*[[Buffer_solution#Common_buffer_compounds_used_in_biology|Common buffer compounds used in biology]]
==References==
{{reflist}}
==External links==
*[http://www.sigmaaldrich.com/life-science/core-bioreagents/biological-buffers/learning-center/buffer-calculator.html Sigma Aldrich Buffer Calculator] - Useful tool to calculate weight, volume, or concentration from molecular weight.
*[http://openwetware.org/index.php?title=MOPS Recipe for MOPS buffer on OpenWetWare]
[[Category:Buffer solutions]]
[[Category:Sulfonic acids]]
[[Category:4-Morpholinyl compounds]]