View source for MeRIPseq

'''MeRIPseq'''<ref>{{cite journal|last=Meyer|first=Kate D.|author2=Saletore, Yogesh |author3=Zumbo, Paul |author4=Elemento, Olivier |author5=Mason, Christopher E. |author6= Jaffrey, Samie R. |title=Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons|journal=Cell|date=31 May 2012|volume=149|issue=7|pages=1635–1646|doi=10.1016/j.cell.2012.05.003|pmid=22608085|pmc=3383396}}</ref> (or '''MeRIP-seq''') stands for [[methylated]] [[RNA immunoprecipitation]] sequencing, which is a method for detection of post-transcriptional [[RNA]] modifications, developed by Kate Meyer et al. while working in the laboratory of Sammie Jaffrey at Cornell University Graduate School of Medical Sciences. It is also called m6A-seq.<ref>{{cite journal|last=Dominissini|first=Dan |author2=Moshitch-Moshkovitz, Sharon |author3=Schwartz, Schraga |author4=Salmon-Divon, Mali |author5=Ungar, Lior |author6=Osenberg, Sivan |author7=Cesarkas, Karen |author8=Jacob-Hirsch, Jasmine |author9=Amariglio, Ninette |author10=Kupiec, Martin |author11=Sorek, Rotem |author12=Rechavi, Gideon |s2cid=3517716 |title=Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq|journal=Nature|date=28 April 2012|volume=485|issue=7397|pages=201–206|doi=10.1038/nature11112|pmid=22575960|bibcode=2012Natur.485..201D }}</ref>
A variation of the MerIP-seq method was coined by Benjamin Delatte and colleagues in 2016. This variant, called hMerIP-seq (hydroxymethylcytosine RNA immunoprecipitation), uses an antibody that specifically recognizes 5-hydroxymethylcytosine, a modified RNA base affecting ''in vitro'' translation and brain development in Drosophila.<ref>{{cite journal|last1=Delatte|first1=Benjamin|title=Transcriptome-wide distribution and function of RNA hydroxymethylcytosine|journal=Science|date=2016|volume=351|issue=6270|pages=282–285|pmid=26816380|doi=10.1126/science.aac5253|bibcode=2016Sci...351..282D|doi-access=free}}</ref>
== References ==
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[[Category:Immunologic tests]]
[[Category:RNA]]