View source for OxyS RNA

{{Infobox rfam
| Name = OxyS RNA
| image = RF00035.jpg
| width =
| caption = Predicted [[secondary structure]] and [[sequence conservation]] of OxyS
| Symbol = OxyS
| AltSymbols =
| Rfam = RF00035
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| RNA_type = [[Gene]]; [[Bacterial small RNA|sRNA]]
| Tax_domain = [[Bacteria]]
| GO =
| SO = {{SO|0000384}}
| CAS_number =
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| OMIM =
| PDB =
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}}
'''OxyS RNA''' is a small [[non-coding RNA]] which is induced in response to oxidative stress in ''[[Escherichia coli]]''. This RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, by an [[antisense]] mechanism, including the fhlA-encoded transcriptional activator and the [[rpoS]]-encoded sigma(s) subunit of RNA polymerase.<ref>{{cite journal | last = Altuvia | first = S |author2=Zhang A |author3=Argaman L |author4=Tiwari A |author5=Storz G | year = 1998 | title = The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding | journal = EMBO J | volume = 17 | pages = 6069–6075 | pmid = 9774350 | doi = 10.1093/emboj/17.20.6069 | issue = 20 | pmc = 1170933}}</ref> OxyS is bound by the [[Hfq protein]], that increases the OxyS RNA interaction with its target messages.<ref>{{cite journal | last = Zhang | first = A |author2=Wassarman KM |author3=Ortega J |author4=Steven AC |author5=Storz G | year = 2002 | title = The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs | journal = Mol Cell | volume = 9 | pages = 11–22 | pmid = 11804582 | doi = 10.1016/S1097-2765(01)00437-3 | issue = 1| doi-access = free }}</ref><ref name="pmid27044921">{{cite journal |vauthors=Holmqvist E, Wright PR, Li L, Bischler T, Barquist L, Reinhardt R, Backofen R, Vogel J |title=Global RNA recognition patterns of post-transcriptional regulators Hfq and CsrA revealed by UV crosslinking in vivo |journal=EMBO J |year=2016 |pmid=27044921 |doi=10.15252/embj.201593360 |volume=35 |issue=9 |pages=991–1011|pmc=5207318 }}</ref> Binding to Hfq alters the conformation of OxyS.<ref>{{cite journal|last=Henderson|first=CA|author2=Vincent, HA |author3=Casamento, A |author4=Stone, CM |author5=Phillips, JO |author6=Cary, PD |author7=Sobott, F |author8=Gowers, DM |author9=Taylor, JE |author10= Callaghan, AJ |title=Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS.|journal=RNA|date=August 2013|volume=19|issue=8|pages=1089–1104|pmid=23804244|doi=10.1261/rna.034595.112 |pmc=3708529}}</ref>  The 109 [[nucleotide]] RNA is thought to be composed of three [[stem-loop]]s.
==Target genes==
A number of additional targets were predicted and verified using microarray analysis.<ref name="pmid16717284">{{cite journal |vauthors=Tjaden B, Goodwin SS, Opdyke JA, etal | title = Target prediction for small, noncoding RNAs in bacteria | journal = Nucleic Acids Res. | volume = 34 | issue = 9 | pages = 2791–2802 | year = 2006 | pmid = 16717284 | doi = 10.1093/nar/gkl356 | pmc = 1464411 }}</ref> These are listed below:
* yobF – confirmed by Northern analysis
* yaiZ
* rumA
* wrbA – confirmed by Northern analysis
* ybaY – confirmed by Northern analysis
Furthermore, the mRNA encoded by the ''nusG'' gene is a direct target of OxyS.<ref name="pmid29237698">{{cite journal |vauthors=Barshishat S, Elgrably-Weiss M, Edelstein J, Georg J, Govindarajan S, Haviv M, Wright PR, Hess WR, Altuvia S |title=OxyS small RNA induces cell cycle arrest to allow DNA damage repair |journal=EMBO J |volume=37 |issue=3 |pages=413–426 |year=2018 |pmid=29237698 |pmc=5793797 |doi=10.15252/embj.201797651}}</ref>
==References==
{{reflist}}
==External links==
* {{Rfam|id=RF00035|name=OxyS RNA}}
* [http://trna.ibch.poznan.pl/ncRNA/scripts/getdesc.cgi?id=OxyS_RNA OxyS entry in the ncRNA database]
[[Category:Non-coding RNA]]
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