View source for SAFB

{{Short description|Protein-coding gene in the species Homo sapiens}}
{{Infobox_gene}}
'''Scaffold attachment factor B''', also known as '''SAFB''', is a [[gene]] with homologs that have been studied in humans and mice.<ref name="entrez">{{cite web | title = Entrez Gene: SAFB scaffold attachment factor B| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6294}}</ref>
== Function ==
This gene encodes a DNA-binding protein that has specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there are conflicting views as to whether this protein is a component of chromatin, the nuclear matrix, or both. Scaffold attachment factors are a subset of nuclear matrix proteins (NMP) with enriched binding  to AT-rich S/MAR sequences. The SAF-B protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.<ref name="entrez" />
== Interactions ==
SAFB has been shown to [[Protein-protein interaction|interact]] with:
* [[Estrogen receptor alpha]],<ref name = pmid15066997>{{cite journal | vauthors = Townson SM, Kang K, Lee AV, Oesterreich S | title = Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a potent transcriptional repression domain | journal = J. Biol. Chem. | volume = 279 | issue = 25 | pages = 26074–81 | date = June 2004 | pmid = 15066997 | doi = 10.1074/jbc.M313726200 | doi-access = free }}</ref><ref name = pmid10707955>{{cite journal | vauthors = Oesterreich S, Zhang Q, Hopp T, Fuqua SA, Michaelis M, Zhao HH, Davie JR, Osborne CK, Lee AV | title = Tamoxifen-bound estrogen receptor (ER) strongly interacts with the nuclear matrix protein HET/SAF-B, a novel inhibitor of ER-mediated transactivation | journal = Mol. Endocrinol. | volume = 14 | issue = 3 | pages = 369–81 | date = March 2000 | pmid = 10707955 | doi = 10.1210/mend.14.3.0432 | doi-access = free }}</ref>
* [[HNRPD]],<ref name = pmid10933876>{{cite journal | vauthors = Arao Y, Kuriyama R, Kayama F, Kato S | title = A nuclear matrix-associated factor, SAF-B, interacts with specific isoforms of AUF1/hnRNP D | journal = Arch. Biochem. Biophys. | volume = 380 | issue = 2 | pages = 228–36 | date = August 2000 | pmid = 10933876 | doi = 10.1006/abbi.2000.1938 }}</ref>
* [[SAFB2]],<ref name = pmid12660241>{{cite journal | vauthors = Townson SM, Dobrzycka KM, Lee AV, Air M, Deng W, Kang K, Jiang S, Kioka N, Michaelis K, Oesterreich S | title = SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor | journal = J. Biol. Chem. | volume = 278 | issue = 22 | pages = 20059–68 | date = May 2003 | pmid = 12660241 | doi = 10.1074/jbc.M212988200 | doi-access = free }}</ref>  and
* [[TAF15]].<ref name = pmid15066997/>
== References ==
{{reflist}}
== Further reading ==
{{refbegin | 2}}
* {{cite journal | vauthors = Oesterreich S | title = Scaffold attachment factors SAFB1 and SAFB2: Innocent bystanders or critical players in breast tumorigenesis? | journal = J. Cell. Biochem. | volume = 90 | issue = 4 | pages = 653–61 | year = 2004 | pmid = 14587024 | doi = 10.1002/jcb.10685 | s2cid = 39178501 }}
* {{cite journal | vauthors = Romig H, Fackelmayer FO, Renz A, Ramsperger U, Richter A | title = Characterization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elements | journal = EMBO J. | volume = 11 | issue = 9 | pages = 3431–40 | year = 1992 | pmid = 1324173 | pmc = 556878 | doi =  10.1002/j.1460-2075.1992.tb05422.x}}
* {{cite journal | vauthors = Renz A, Fackelmayer FO | title = Purification and molecular cloning of the scaffold attachment factor B (SAF-B), a novel human nuclear protein that specifically binds to S/MAR-DNA | journal = Nucleic Acids Res. | volume = 24 | issue = 5 | pages = 843–9 | year = 1996 | pmid = 8600450 | pmc = 145707 | doi = 10.1093/nar/24.5.843 }}
* {{cite journal | vauthors = Oesterreich S, Lee AV, Sullivan TM, Samuel SK, Davie JR, Fuqua SA | title = Novel nuclear matrix protein HET binds to and influences activity of the HSP27 promoter in human breast cancer cells | journal = J. Cell. Biochem. | volume = 67 | issue = 2 | pages = 275–86 | year = 1997 | pmid = 9328833 | doi = 10.1002/(SICI)1097-4644(19971101)67:2<275::AID-JCB13>3.0.CO;2-E | s2cid = 29247141 }}
* {{cite journal | vauthors = DuPont BR, Garcia DK, Sullivan TM, Naylor SL, Oesterreich S | title = Assignment of SAFB encoding Hsp27 ERE-TATA binding protein (HET)/scaffold attachment factor B (SAF-B) to human chromosome 19 band p13 | journal = Cytogenet. Cell Genet. | volume = 79 | issue = 3–4 | pages = 284–5 | year = 1998 | pmid = 9605873 | doi = 10.1159/000134744 }}
* {{cite journal | vauthors = Nayler O, Strätling W, Bourquin JP, Stagljar I, Lindemann L, Jasper H, Hartmann AM, Fackelmayer FO, Ullrich A, Stamm S | title = SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements | journal = Nucleic Acids Res. | volume = 26 | issue = 15 | pages = 3542–9 | year = 1998 | pmid = 9671816 | pmc = 147731 | doi = 10.1093/nar/26.15.3542 }}
* {{cite journal | vauthors = Weighardt F, Cobianchi F, Cartegni L, Chiodi I, Villa A, Riva S, Biamonti G | title = A novel hnRNP protein (HAP/SAF-B) enters a subset of hnRNP complexes and relocates in nuclear granules in response to heat shock | journal = J. Cell Sci. | volume = 112 | issue = 10 | pages = 1465–76 | year = 1999 | doi = 10.1242/jcs.112.10.1465 | pmid = 10212141 }}
* {{cite journal | vauthors = Oesterreich S, Zhang Q, Hopp T, Fuqua SA, Michaelis M, Zhao HH, Davie JR, Osborne CK, Lee AV | title = Tamoxifen-bound estrogen receptor (ER) strongly interacts with the nuclear matrix protein HET/SAF-B, a novel inhibitor of ER-mediated transactivation | journal = Mol. Endocrinol. | volume = 14 | issue = 3 | pages = 369–81 | year = 2000 | pmid = 10707955 | doi = 10.1210/mend.14.3.0432 | doi-access = free }}
* {{cite journal | vauthors = Shnyreva M, Schullery DS, Suzuki H, Higaki Y, Bomsztyk K | title = Interaction of two multifunctional proteins. Heterogeneous nuclear ribonucleoprotein K and Y-box-binding protein | journal = J. Biol. Chem. | volume = 275 | issue = 20 | pages = 15498–503 | year = 2000 | pmid = 10809782 | doi = 10.1074/jbc.275.20.15498 | doi-access = free }}
* {{cite journal | vauthors = Arao Y, Kuriyama R, Kayama F, Kato S | title = A nuclear matrix-associated factor, SAF-B, interacts with specific isoforms of AUF1/hnRNP D | journal = Arch. Biochem. Biophys. | volume = 380 | issue = 2 | pages = 228–36 | year = 2000 | pmid = 10933876 | doi = 10.1006/abbi.2000.1938 }}
* {{cite journal | vauthors = Stoss O, Olbrich M, Hartmann AM, Konig H, Memmott J, Andreadis A, Stamm S | title = The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites | journal = J. Biol. Chem. | volume = 276 | issue = 12 | pages = 8665–73 | year = 2001 | pmid = 11118435 | doi = 10.1074/jbc.M006851200 | doi-access = free }}
* {{cite journal | vauthors = Oesterreich S, Allredl DC, Mohsin SK, Zhang Q, Wong H, Lee AV, Osborne CK, O'Connell P | title = High rates of loss of heterozygosity on chromosome 19p13 in human breast cancer | journal = Br. J. Cancer | volume = 84 | issue = 4 | pages = 493–8 | year = 2001 | pmid = 11207044 | pmc = 2363776 | doi = 10.1054/bjoc.2000.1606 }}
* {{cite journal | vauthors = Nikolakaki E, Kohen R, Hartmann AM, Stamm S, Georgatsou E, Giannakouros T | title = Cloning and characterization of an alternatively spliced form of SR protein kinase 1 that interacts specifically with scaffold attachment factor-B | journal = J. Biol. Chem. | volume = 276 | issue = 43 | pages = 40175–82 | year = 2001 | pmid = 11509566 | doi = 10.1074/jbc.M104755200 | doi-access = free }}
* {{cite journal | vauthors = Paul C, Manero F, Gonin S, Kretz-Remy C, Virot S, Arrigo AP | title = Hsp27 as a Negative Regulator of Cytochrome c Release | journal = Mol. Cell. Biol. | volume = 22 | issue = 3 | pages = 816–34 | year = 2002 | pmid = 11784858 | pmc = 133538 | doi = 10.1128/MCB.22.3.816-834.2002 }}
* {{cite journal | vauthors = Traweger A, Fuchs R, Krizbai IA, Weiger TM, Bauer HC, Bauer H | title = The tight junction protein ZO-2 localizes to the nucleus and interacts with the heterogeneous nuclear ribonucleoprotein scaffold attachment factor-B | journal = J. Biol. Chem. | volume = 278 | issue = 4 | pages = 2692–700 | year = 2003 | pmid = 12403786 | doi = 10.1074/jbc.M206821200 | doi-access = free }}
* {{cite journal | vauthors = Townson SM, Dobrzycka KM, Lee AV, Air M, Deng W, Kang K, Jiang S, Kioka N, Michaelis K, Oesterreich S | title = SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor | journal = J. Biol. Chem. | volume = 278 | issue = 22 | pages = 20059–68 | year = 2003 | pmid = 12660241 | doi = 10.1074/jbc.M212988200 | doi-access = free }}
* {{cite journal | vauthors = van den IJssel P, Wheelock R, Prescott A, Russell P, Quinlan RA | title = Nuclear speckle localisation of the small heat shock protein alpha B-crystallin and its inhibition by the R120G cardiomyopathy-linked mutation | journal = Exp. Cell Res. | volume = 287 | issue = 2 | pages = 249–61 | year = 2003 | pmid = 12837281 | doi = 10.1016/S0014-4827(03)00092-2 }}
* {{cite journal | vauthors = Tai HH, Geisterfer M, Bell JC, Moniwa M, Davie JR, Boucher L, McBurney MW | title = CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins | journal = Biochem. Biophys. Res. Commun. | volume = 308 | issue = 1 | pages = 170–6 | year = 2003 | pmid = 12890497 | doi = 10.1016/S0006-291X(03)01354-8 }}
* {{cite journal | vauthors = Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG | title = Regulation of Alternative Splicing by SRrp86 and Its Interacting Proteins | journal = Mol. Cell. Biol. | volume = 23 | issue = 21 | pages = 7437–47 | year = 2003 | pmid = 14559993 | pmc = 207616 | doi = 10.1128/MCB.23.21.7437-7447.2003 }}
{{refend}}
{{gene-19-stub}}