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Jelena Janković defeated Dinara Safina in the final, 6–4, 6–2 to win the women's singles tennis title at the 2009 Cincinnati Masters.
Nadia Petrova was the defending champion, but lost in the first round to Alona Bondarenko.
This tournament marked the return of Kim Clijsters from the sport after her first retirement, having not played a match since May 2007. She advanced to the quarterfinals, where she was beaten by Safina.